《植物生理学报》 2018, 54(4): 660-668

叶面喷施褪黑素对小白菜幼苗镉耐性的影响

刘自力, 黄一凡, 朱正波, 陈亚华, 崔瑾^*

南京农业大学生命科学学院, 南京210095

通信作者：崔；E-mail: cuijin@njau.edu.cn

摘　要：

以小白菜‘矮脚黄’为试验材料, 探究叶面喷施褪黑素(100 μmol·L^-1)对小白菜幼苗Cd胁迫(20 μmol·L^-1)耐性的影响。试验测定小白菜幼苗生物量和生理指标变化, 以及Cd吸收和转运相关基因转录水平。结果表明, 叶面喷施褪黑素能有效缓解Cd胁迫对小白菜幼苗生长造成的抑制, 并伴随着叶绿素含量以及光合参数的提高, 显著降低活性氧(O₂ ^¯·、H₂O₂)和MDA的含量, 提高抗氧化酶(SOD、CAT、POD、APX)的活性和抗氧化物质(AsA、GSH)的含量, 并且显著降低小白菜幼苗中Cd的含量。进一步研究发现, 叶面喷施褪黑素能够显著降低IRT1、IRT2、Nramp1、Nramp3、HMA2、HMA4的转录水平。综上所述, 叶面喷施褪黑素能够清除Cd胁迫下小白菜幼苗中过量积累的活性氧, 缓解氧化胁迫, 并通过影响小白菜Cd吸收和转运相关基因的转录来降低小白菜幼苗对Cd的积累, 提高Cd耐性。

关键词：褪黑素; 镉; 抗氧化作用; 小白菜; 转运系数

收稿：2018-03-01   修定：2018-03-15

资助：国家重点研发计划(2016YFD0800700-3)和现代农业产业技术体系建设专项(CARS-23-A03)。

Effects of foliar feeding of melatonin on cadmium tolerance of Chinese cabbage seedlings

LIU Zi-Li, HUANG Yi-Fan, ZHU Zheng-Bo, CHEN Ya-Hua, CUI Jin^*

College of Life Science, Nanjing Agricultural University, Nanjing 210095, China

Corresponding author: CUI Jin; E-mail: cuijin@njau.edu.cn

Abstract:

Potted Chinese cabbage cultivar ‘Aijiaohuang’ were used to study the effects of foliar feeding of melatonin (100 μmol·L^-1) on cadmium tolerance of Chinese cabbage seedlings under cadmium (20 μmol·L^-1) stress. Biomass and physiological indices were determinated, and transcription of Cd absorption and translocation related genes were tested, too. The results showed that foliar feeding of melatonin significantly alleviated the Cd toxic symptoms, including the improvement of both biomass and chlorophyll content. These responses were consistent with a significant decrease of ROS (O₂ ^¯·, H₂O₂) and MDA. Melatonin enhanced the activities of antioxidant enzymes (SOD, CAT, POD, APX) and content of antioxidant substance (AsA, GSH), and significantly decreased Cd accumulation of roots and shoots in Chinese cabbage seedlings. Furthermore, IRT1, IRT2, Nramp1, Nramp3, HMA2, HMA4<、i> were blocked by melatonin. Therefore, foliar feeding of melatonin could scavenge active oxygen to alleviate the effect of growth inhibition on Chinese cabbage seedlings caused by Cd stress. Meanwhile, melatonin may enhance Cd tolerance of Chinese cabbage seedlings by reducing expression of genes which restrained Cd uptake and accumulation.

Key words: melatonin; cadmium; antioxidation; Chinese cabbage; translocation factor

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